Pig Anti-Mullerian Hormone (MIS;AMH) ELISA Kit

Cat.#:

JL17804

Regulatory Status:

RUO

Alternative:

MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

78.12-5000pg/mL

Sensitivity:

34.76pg/mL

Specificity:

This kit recognizes Pig MIS;AMH in samples. No significant cross-reactivity or interference between Pig MIS;AMH and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Developmental Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| MIS;AMH

Anti-Müllerian hormone (AMH), also known as Müllerian-inhibiting hormone (MIH), is a glycoprotein hormone structurally related to inhibin and activin from the transforming growth factor beta superfamily, whose key roles are in growth differentiation and folliculogenesis.AMH is a dimeric glycoprotein with a molar mass of 140 kDa.The molecule consists of two identical subunits linked by sulfide bridges, and characterized by the N-terminal dimer (pro-region) and C-terminal dimer.AMH binds to its Type 2 receptor AMHR2, which phosphorylates a type I receptor under the TGF beta signaling pathway.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Pig MIS;AMH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-102	95
Plasma(n=8)	94-107	104
Cell Culture Supernates(n=8)	96-112	104

| Linearity

Samples were spiked with high concentrations of Pig MIS;AMH and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	82-95	88-96	90-110
1 : 2	Average (%)	91	91	97
1 : 4	Range (%)	89-103	87-110	105-115
1 : 4	Average (%)	94	98	109